Intracellular ion imaging using fluorescent dyes: artefacts and limits to resolution

Abstract

Development of highly efficient fluorescent ratio indicators has made imaging of ion concentrations within individual cells possible (Grynkiewicz et al. 1985; Tsien and Poenie 1986). Ion imaging is a complex technique and is therefore prone to artefacts. In this paper we investigate the limits of the technique and its potential pitfalls. The spatial… (More)
DOI: 10.1007/BF00374639

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