Intracellular ice formation in mouse oocytes subjected to interrupted rapid cooling.

@article{Mazur2007IntracellularIF,
  title={Intracellular ice formation in mouse oocytes subjected to interrupted rapid cooling.},
  author={P{\'e}ter Mazur and Irina L Pinn and Frederick William Kleinhans},
  journal={Cryobiology},
  year={2007},
  volume={55 2},
  pages={
          158-66
        }
}
The formation of ice crystals within cells (IIF) is lethal. The classical approach to avoiding it is to cool cells slowly enough so that nearly all their supercooled freezable water leaves the cell osmotically before they have cooled to a temperature that permits IIF. An alternative approach is to cool the cell rapidly to just above its ice nucleation temperature, and hold it there long enough to permit dehydration. Then, the cell is cooled rapidly to -70 degrees C or below. This approach… CONTINUE READING
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