Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1.

  title={Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1.},
  author={S T Smiley and Martin Reers and C Mottola-Hartshorn and M Lin and A B Chen and T. W. Smith and Glenn D. Steele and Li Ban Chen},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  pages={3671 - 3675}
  • S. Smiley, M. Reers, +5 authors L. B. Chen
  • Published 1 May 1991
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
By using a potential-dependent J-aggregate-forming delocalized lipophilic cation, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine++ + iodide (JC-1), we find that membrane potentials across mitochondria in a living cell can be heterogeneous. Remarkably, even within a long contiguous mitochondrion, regional heterogeneity in membrane potentials appears to be possible. 
Labeling mitochondria with JC-1.
  • B. Chazotte
  • Biology, Medicine
  • Cold Spring Harbor protocols
  • 2011
This protocol describes the labeling of mitochondria in cultured cells with JC-1, which has been useful in flow-cytometry studies, because the membrane potential can be followed without the need for confocal microscopy. Expand
Do single mitochondria contain zones with different membrane potential
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Mitochondrial membrane potential in single living adult rat cardiac myocytes exposed to anoxia or metabolic inhibition.
JC‐1 represents a unique double sensor which can provide semi‐quantitative information in both low and high potential ranges, suggesting that delta psi m, instead of producing ATP, is generated by glycolytic ATP during anoxia. Expand
Assessment of mitochondrial membrane potential in proximal tubules after hypoxia-reoxygenation.
The behavior of 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazocarbocyanine iodide (JC-1) is investigated and a more dynamic and quantitative approach employing safranin O for use with the tubule system is introduced. Expand
Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe.
The data analysis of mitochondrial membrane potential is one associated with the impact of xenobiotics such as tobacco smoking on blood-brain barrier endothelial cells including both mouse primary (mBMEC) and a mouse-based endothelial cell line (bEnd3) in a side by side comparison. Expand
Flow Cytometric Analysis of Mitochondrial Membrane Potential Using JC‐1
This unit presents very recent developments in both fluorescent probes and functional applications and demonstrates the use of the JC‐1 probe for measuring mitochondrial membrane potential by flow cytometry. Expand
Mitochondrial membrane charge differentiation in areas of axonal overlap and in areas of cell bodies using JC-1, a J-aggregate vital fluorescent dye.
Higher charge in areas of axonal overlap may be due to the fact that there is a higher mitochondrial membrane potential in areas where highenergy expenditure is taking place, and these areas are interacting with each other thus; need energy to allow this phenomenon to occur. Expand
Fluorometric Methods for Detection of Mitochondrial Membrane Depolarization Induced by CD95 Activation.
This chapter reports how the drop of the mitochondrial transmembrane potential in leukemic cells and adherent triple negative breast cancer cells exposed to cytotoxic CD95L is evaluated using classical fluorescent probes, DIOC6(3) and TMRM. Expand
Ethanol hyperpolarizes mitochondrial membrane potential and increases mitochondrial fraction in cultured mouse myocardial cells
Myocyte-specific alterations indicate an increase in the mitochondrial fraction in a cell, which might be a countermeasure against ethanol-induced dysfunction of mitochondrial respiration that is needed to meet the energy requirements of spontaneous myocardial contractions. Expand
Estimation of Membrane Potential by Flow Cytometry
  • H. Shapiro
  • Medicine, Biology
  • Current protocols in cytometry
  • 2004
This unit provides methods for accomplishing this goal by flow or static cytometry using any of a number of cationic or anionic lipophilic fluorescent probes known as distributional dyes, able to estimate the membrane potential of eukaryotic or bacterial cells. Expand