Intracellular flux of glucose metabolism in streptococcal cells by simultaneous monitoring of fluorescence dependent on reduced nicotinamide adenine nucleotide and acid excretion under strictly anaerobic conditions.

Reduced nicotinamide adenine nucleotide (NADH)-dependent fluorescence and acid excretion during glucose pulse to washed Streptococcus mutans cells were monitored simultaneously at pH 7.0 with a fluorescence spectrophotometer and a pH-stat. Acid excretion started with addition of glucose. At the same time, the fluorescence dropped quickly to a minimum level… CONTINUE READING