Corpus ID: 19568463

Intracellular fate of a multivalent ligand covalently bound to cell surface components. An electron microscopic study.

@article{Murean1987IntracellularFO,
  title={Intracellular fate of a multivalent ligand covalently bound to cell surface components. An electron microscopic study.},
  author={V. Mureşan},
  journal={Journal of submicroscopic cytology},
  year={1987},
  volume={19 3},
  pages={
          375-85
        }
}
  • V. Mureşan
  • Published 1987
  • Biology, Medicine
  • Journal of submicroscopic cytology
The electron dense tracer ferritin hydrazide (FH) was covalently attached to sialoglycoconjugates of the luminal surface of capillary endothelium in rat pancreas, after chemical modification of sialyl residues. Mild oxidation of the vasculature by perfusion with 1 mM sodium periodate was followed by a 30-90 min incubation with the tracer, at 37 degrees C. More than 60% of coated pits and coated vesicles were marked by FH, although in only approximately 25% of these structures the label density… Expand
2 Citations
High and low molecular weight tracers for the electron microscopical detection of sialoglycoconjugates
TLDR
Results indicate that the three hydrazide-derivative tracers may be useful tools for the electron microscopical detection of cellular and extracellular sialoglycoconjugates. Expand
Early acinar cell changes in caerulein-induced interstitial acute pancreatitis in the rat.
TLDR
Early ultrastructural and immunohistochemical changes caused by supramaximal secretory stimulation with caerulein were studied in the rat pancreas and found that the pancreozymin-receptor antagonist proglumide as well as cycloleucine that inhibits protein synthesis by inhibiting the synthesis of S-adenosylmethionine effectively prevented the caerulesin induced acinar cell changes. Expand