Intracellular cytokine staining for the characterization and quantitation of antigen-specific T lymphocyte responses.

@article{Gauduin2006IntracellularCS,
  title={Intracellular cytokine staining for the characterization and quantitation of antigen-specific T lymphocyte responses.},
  author={M C Gauduin},
  journal={Methods},
  year={2006},
  volume={38 4},
  pages={263-73}
}
Standard proliferation assays used for analysis of T cell function have significant shortcomings, including limited sensitivity, lack of quantitative readouts, and considerable variability. Recently, flow cytometric methods have been developed to allow multiparametric detection of cell surface antigens and intracellular cytokine expression in response to polyclonal stimuli and antigen. We have optimized an intracellular cytokine staining assay in the non-human primate model of AIDS, which… CONTINUE READING
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