Intracellular colonization of roots of Arabidopsis and crop plants by Gluconacetobacter diazotrophicus

  title={Intracellular colonization of roots of Arabidopsis and crop plants by Gluconacetobacter diazotrophicus},
  author={Edward C. Cocking and Philip J. Stone and Michael Raymond Davey},
  journal={In Vitro Cellular \& Developmental Biology - Plant},
SummaryWe have investigated the interaction of Gluconacetobacter diazotrophicus, a non-nodulating endophytic nitrogen-fixing bacterium isolated from the intercellular spaces of sugarcane, with Arabidopsis thaliana and the crop plants maize (Zea mays), rice (Oryza sativa), wheat (Triticum aestivum), oilseed rape (Brassica napus), tomato (Lycopersicon esculentum), and white clover (Trifolium repens). Using seedlings grown aseptically in sucrose-containing culture media, we have shown that… 
Endophytic Colonization of tomato plants by Gluconacetobacter diazotrophicus and its effect on crops improvement and yield promotion
Results showed that coating tomato seeds with specificconcentrations of G.d-Nfix-3%sucrose was an effective enhancer for crop production at low nitrogen levels and proved the ability ofG.d to enhance production of tomato fruits.
Endophytic colonization of Arabidopsis thaliana by Gluconacetobacter diazotrophicus and its effect on plant growth promotion, plant physiology, and activation of plant defense
The inoculation of plants defective in systemic acquired resistance (SAR)-associated defense revealed that plant immune system plays an important role during the early association stages, and indicates that A. thaliana is useful for molecular studies of the mechanisms involved in the interaction between plants and PGPB, especially those involving G. diazotrophicus.
Colonization of Brachypodium distachyon by Gluconacetobacter diazotrophicus and its effect on plant growth promotion
Abstract: Gluconacetobacter diazotrophicus is a nitrogen (N) fixing bacterium originally isolated from sugarcane in Brazil. Understanding the interactions between this bacterium and plants is
Colonization of the nitrogen-fixing bacterium Gluconacetobacter diazotrophicus in a large number of Canadian corn plants.
The research showed that it was possible to introduce G. diazotrophicus into a large number of corn genotypes and the bacterium showed a positive adaptation to the new host plant.
Growth promotion of rice seedlings by Gluconacetobacter diazotrophicus under in vitro conditions
It was found that this strain could colonize the roots of paddy when inoculated in the growth solution under aseptic hydroponic condition in a specially designed beaker and colonization of this introduced strain in the paddy roots was confirmed by performing indirect fluorescent antibody reaction using antiserum.
OBPC Symposium: Maize 2004 & beyond—Intracellular colonization of cereals and other crop plants by nitrogen-fixing bacteria for reduced inputs of synthetic nitrogen fertilizers
  • E. Cocking
  • Biology
    In Vitro Cellular & Developmental Biology - Plant
  • 2007
The extent to which nitrogen fixation is correlated with systemic intracellular colonization by G. diazotrophicus is determined, to obtain an indication of the likelihood of adequate growth and yield of maize with reduced inputs of synthetic nitrogen fertilizers.
Ecological Occurrence of Gluconacetobacter diazotrophicus and Nitrogen-fixing Acetobacteraceae Members: Their Possible Role in Plant Growth Promotion
The association of G. diazotrophicus with sugarcane, other crop plants and with various hosts is discussed and the plant-growth-promoting traits identified in this group of bacteria, including N2 fixation, phytohormone synthesis, P and Zn solubilization and biocontrol, are analysed.
Mitigating Increases in Nitrogen Deposition: The Challenge of Extending Symbiotic Nitrogen Fixation to Cereals and Other Non-legume Crops
In inoculating seedlings of maize, rice, wheat, oilseed rape and tomato grown aseptically in sucrose-containing culture media with very low numbers of Gluconacetobacter diazotrophicus it is shown that this N-fixing bacterium is able to intracellularly colonise the meristematic cells of their roots.
The Intracellular Scots Pine Shoot Symbiont Methylobacterium extorquens DSM13060 Aggregates around the Host Nucleus and Encodes Eukaryote-Like Proteins
A novel endophytic niche is described inside cells of the dividing shoot tissues of a pine and stimulates seedling growth without producing cytokinin, auxin, or other plant hormones commonly synthesized by plant-associated bacteria.


Further observations on the interaction between sugar cane and Gluconacetobacter diazotrophicus under laboratory and greenhouse conditions.
Sugar cane (Saccharum spp.) variety SP 70-1143 was inoculated with Gluconacetobacter diazotrophicus strain PAL5 (ATCC 49037) in two experiments, and there was extensive anatomical evidence of endophytic colonization by G. diaztrophicus.
Infection and colonization of rice seedlings by the plant growth-promoting bacterium Herbaspirillum seropedicae Z67.
A beta-glucoronidase (GUS)-marked strain of Herbaspirillum seropedicae Z67 was inoculated onto rice seedling cvs. IR42 and IR72. Internal populations peaked at over 10(6) log CFU per gram of fresh
Expression of sugarcane genes induced by inoculation with Gluconacetobacter diazotrophicus and Herbaspirillum rubrisubalbicans
An inventory of sugarcane genes, candidates for exclusive or preferential expression during the nitrogen-fixing association suggests that the host plant might be actively involved in the establishment of the interaction with G. diazotrophicus and H. rubrisubalbicans.
Comparison of benefit to sugarcane plant growth and 15N2 incorporation following inoculation of sterile plants with Acetobacter diazotrophicus wild-type and Nif- mutants strains.
Results indicate that the transfer of fixed N from A. diazotrophicus to sugarcane might be a significant mechanism for plant growth promotion in this association.
Azorhizobium caulinodans ORS571 colonizes the xylem of Arabidopsis thaliana.
Improved conditions were used for the aseptic growth of Arabidopsis thaliana to investigate whether xylem colonization by Azorhizobium caulinodans ORS571 might occur, and detailed microscopic analysis of sections of primary and lateral roots from inoculated A.Thaliana confirmed xylesm colonization.
Gluconacetobacter diazotrophicus (syn. Acetobacter diazotrophicus), a promising diazotrophic endophyte in tropics
The main objective of this review is to summarize the recent works on G. diazotrophicus with special emphasis on its interaction with sugarcane.
A Nitrogen-Fixing Endophyte of Sugarcane Stems (A New Role for the Apoplast)
This demonstrates the presence of an N2-fixing endophyte living in apoplastic fluid of plant tissue and also that the fluid approximates the composition of the endophytes's optimal culture medium.
Intracellular accommodation of microbes by plants: a common developmental program for symbiosis and disease?
  • M. Parniske
  • Biology, Environmental Science
    Current opinion in plant biology
  • 2000
Nitrogen fixation in wheat provided by Klebsiella pneumoniae 342.
All of the criteria necessary for the demonstration of nitrogen fixation in wheat are shown upon inoculation with a nitrogen-fixing bacterium, Klebsiella pneumoniae 342 (Kp342), and Kp342 relieved nitrogen (N) deficiency symptoms and increased total N and N concentration in the plant.