Intracellular calcium regulation in sperm capacitation and acrosomal reaction

@article{Breitbart2002IntracellularCR,
  title={Intracellular calcium regulation in sperm capacitation and acrosomal reaction},
  author={Haim Breitbart},
  journal={Molecular and Cellular Endocrinology},
  year={2002},
  volume={187},
  pages={139-144}
}
  • H. Breitbart
  • Published 22 February 2002
  • Biology
  • Molecular and Cellular Endocrinology

Inhibition of Capacitation-Associated Tyrosine Phosphorylation Signaling in Rat Sperm by Epididymal Protein Crisp-11

TLDR
Capacitation results in the acquisition of hyperactivated motility, changes in the properties of the plasma membrane, including changes in proteins and glycoproteins, and acquisition of the ability to undergo the acrosome reaction.

Protein kinase C activity in boar sperm

TLDR
The results presented here reveal a participation of PKC in sperm motility regulation and membrane fluidity changes, which is probably associated to acrosome reaction and to agglutination, and show the existence of a hierarchy in the kinases pathway.

The BSA-induced Ca(2+) influx during sperm capacitation is CATSPER channel-dependent

TLDR
The initial phase of the increases in intracellular calcium concentration induced by BSA requires the CATSPER channel, but not the voltage-gated calcium channel, which is the molecular conduit responsible for the calcium entry required for the sperm motility changes that occur during capacitation.

Localization of guanylate cyclase receptor, inositol trisphosphate receptor, and calmodulin in boar spermatozoa.

TLDR
Using immunolocalization procedures, it is reported that guanylate cyclase receptors (GCR) are localized on the apical region of the acrosome and on the postacrosomal plasmalemma, while IP3Rs are present in the neck area of the sperm and at lower density along the axonemal membrane.

Phospholipase Cδ4 is required for Ca2+ mobilization essential for acrosome reaction in sperm

Zona pellucida (ZP)–induced acrosome reaction in sperm is a required step for mammalian fertilization. However, the precise mechanism of the acrosome reaction remains unclear. We previously reported

PKA activation in concert with ARIS and asterosap induces the acrosome reaction in starfish

TLDR
Results suggest that PKA activity participates in the induction of the AR with ARIS and asterosap, and cloned a gene that encodes a regulatory subunit of PKA that had been identified in starfish spermatozoa.

Delineation of downstream signalling components during acrosome reaction mediated by heat solubilized human zona pellucida

TLDR
Results described in the present study provide a comprehensive account of the various downstream signalling components associated with human ZP mediated acrosome reaction.

Calcium Influx and Male Fertility in the Context of the Sperm Proteome: An Update

TLDR
This review synthesized the published findings on the Ca2+ influx mechanism in mammalian spermatozoa and its implications for the regulation of male fertility in the context of sperm proteins to provide further insight into understanding the Ca1+ influx, protein functions, and regulation of fertility.
...

References

SHOWING 1-10 OF 43 REFERENCES

The biochemistry of the acrosome reaction.

TLDR
It is suggested that ZP binds to at least two different receptors in the plasma membrane, one of which is a Gi-coupled receptor that activates phospholipase C (PLC) beta 1 and the other a tyrosine kinase receptor coupled to PLC gamma.

Inositol 1,4,5-trisphosphate receptors selectively localized to the acrosomes of mammalian sperm

TLDR
Inositol 1,4,5-trisphosphate (IP3)-gated calcium channels and upstream components of the phosphoinositide signaling system in mammalian sperm are characterized and thapsigargin, a sequiterpene lactone inhibitor of the microsomal Ca(2+)-ATPase, stimulates the acrosome reaction of mouse sperm.

Activation of mouse sperm phosphatidylinositol‐4,5 bisphosphate‐phospholipase C by zona pellucida is modulated by tyrosine phosphorylation

TLDR
It is reported that stimulation with solubilized zp increased PIP2‐PLC enzymatic activity from mouse sperm, suggesting that PLCγ1 constitutes a component in the cascade that couples sperm binding to the egg's extracellular matrix with acrosomal exocytosis, a regulated secretory response upon which fertilization depends absolutely.

Calcium mobilization and influx during sperm exocytosis.

TLDR
It is reported using bovine sperm that this bacterial PLC can restore actin release from extracted membranes as well as membrane fusion in a cell-free assay when the endogenous PLC is inhibited by neomycin.

The mammalian acrosome reaction: Gateway to sperm fusion with the oocyte?

  • C. AllenD. Green
  • Biology
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1997
TLDR
The role of the acrosome reaction in the development of fusion competence in sperm is focused on and may throw light on the mechanism of other intercellular fusions such as myoblast fusion, and the evolutionary relationship of inter cellular membrane fusion to intracellular membrane fusion.

Relationship between calcium binding sites and membrane fusion during the acrosome reaction induced by lonophore in ram spermatozoa

Ram spermatozoa treated with the ionophore A23187, to induce the acrosome reaction, were fixed in pyroantimonate-osmium in order to demonstrate calcium at an ultrastructural level. Prior to

Initiation of the human sperm acrosome reaction by thapsigargin.

TLDR
It is shown that thapsigargin, a highly specific inhibitor of the endoplasmic reticulum Ca(2+)-ATPase Ca( 2+)-pump (and thus a mobilizer of intracellular Ca2+) in other cells, can initiate the AR in capacitated human sperm.

Sequential focal and global elevations of sperm intracellular Ca2+ are initiated by the zona pellucida during acrosomal exocytosis.

TLDR
A model is proposed in which ZP3 initiates a G protein-independent opening of the cation channel, producing depolarization of sperm membrane potential and consequent opening of L channels, which promotes acrosomal exocytosis.