Intracellular calcium measurements in individual human sperm demonstrate that the majority can respond to progesterone.

@article{Plant1995IntracellularCM,
  title={Intracellular calcium measurements in individual human sperm demonstrate that the majority can respond to progesterone.},
  author={Ashley S Plant and Eileen A McLaughlin and W. Chris L. Ford},
  journal={Fertility and sterility},
  year={1995},
  volume={64 6},
  pages={1213-5}
}
OBJECTIVE To determine if human spermatozoa could be immobilized and intracellular calcium measurements made on individual cells to measure what proportion can respond to P. DESIGN Spermatozoa were loaded with Fura 2 (Sigma Chemical Co., Poole, Dorest, United Kingdom) and suspended in 10% gelatin at 37 degrees C. A thin layer of the suspension was cooled to room temperature (20 degrees C to 25 degrees C) and [Ca2+]i was measured with a fluorescence microscope equipped with dual wavelength… CONTINUE READING

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