Intracellular antibody capture technology: application to selection of intracellular antibodies recognising the BCR-ABL oncogenic protein.

@article{Tse2002IntracellularAC,
  title={Intracellular antibody capture technology: application to selection of intracellular antibodies recognising the BCR-ABL oncogenic protein.},
  author={Eric Tse and M Natividad Lobato and Alan Michael Forster and Tomoyuki N. Tanaka and Grace T-Y Chung and Terence H Rabbitts},
  journal={Journal of molecular biology},
  year={2002},
  volume={317 1},
  pages={
          85-94
        }
}
The expression of antibodies inside cells to ablate protein function has the potential for disease therapy and for target validation in functional genomics. However, due to inefficient expression or folding, only a few antibodies or antibody fragments, usually as single-chain Fv antibody fragments (scFv), bind their antigens in an intracellular environment. We have established a genetic-selection technology (intracellular antibody capture, IAC) to facilitate the isolation of functional… CONTINUE READING
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