Intracellular PHB conversion in a Type II methanotroph studied by (13)C NMR.

@article{Vecherskaya2001IntracellularPC,
  title={Intracellular PHB conversion in a Type II methanotroph studied by (13)C NMR.},
  author={Margarita Vecherskaya and Cor Dijkema and Alfons J M Stams},
  journal={Journal of industrial microbiology & biotechnology},
  year={2001},
  volume={26 1/2},
  pages={15-21}
}
Poly-beta-hydroxybutyrate (PHB) formation under aerobic conditions via incorporation of [(13)C-2]acetate as a cosubstrate and its intracellular degradation under anaerobic conditions in a Type II methanotroph was studied by (13)C NMR. During PHB synthesis in the presence of labelled acetate, low levels of beta-hydroxybutyrate, butyrate, acetone, isopropanol, 2,3-butanediol and succinate were observed. Subsequent anaerobic PHB breakdown showed enhanced levels of these products at the expense of… CONTINUE READING