Intracellular Distribution and Mechanism of Delivery of Oligonucleotides Mediated by Cationic Lipids

@article{Zelphati2004IntracellularDA,
  title={Intracellular Distribution and Mechanism of Delivery of Oligonucleotides Mediated by Cationic Lipids},
  author={Olivier Zelphati and Francis C. Szoka},
  journal={Pharmaceutical Research},
  year={2004},
  volume={13},
  pages={1367-1372}
}
AbstractPurpose. To study the parameters influencing the intracellular trafficking of oligonucleotides delivered by cationic 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) lipids and to elucidate the mechanism of uptake. Methods. We have studied the intracellular localization of fluorescently labeled oligonucleotide (F-ODN) delivered by DOTAP using confocal microscopy and measured inhibition of luciferase synthesis. The delivery mechanism of ODN/DOTAP complexes was investigated using… 
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