Intracellular Detection and Evolution of Site-Specific Proteases Using a Genetic Selection System

@article{Verhoeven2012IntracellularDA,
  title={Intracellular Detection and Evolution of Site-Specific Proteases Using a Genetic Selection System},
  author={Kathryn Denise Verhoeven and Olvia C Altstadt and Sergey N. Savinov},
  journal={Applied Biochemistry and Biotechnology},
  year={2012},
  volume={166},
  pages={1340-1354}
}
Development of endoproteases, programmed to promote degradation of peptides or proteins responsible for pathogenic states, represents an attractive therapeutic strategy, since such biocatalytic agents could be directed against a potentially unlimited repertoire of extracellular proteinaceous targets. Difficulties associated with engineering enzymes with tailor-made substrate specificities have, however, hindered the discovery of proteases possessing both the efficiency and selectivity to act as… Expand

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