Intracellular Availability of pDNA and mRNA after Transfection: A Comparative Study among Polyplexes, Lipoplexes, and Lipopolyplexes.

@article{Gonalves2016IntracellularAO,
  title={Intracellular Availability of pDNA and mRNA after Transfection: A Comparative Study among Polyplexes, Lipoplexes, and Lipopolyplexes.},
  author={Cristine Gonçalves and Sohail Akhter and Chantal Pichon and Patrick Midoux},
  journal={Molecular pharmaceutics},
  year={2016},
  volume={13 9},
  pages={
          3153-63
        }
}
Intracellular availability of nucleic acids from synthetic vectors is critical and directly influences the transfection efficiency (TE). Herein, we evaluated the TE of polymer- and lipid-based nanoplexes (polyplexes, lipoplexes and lipopolyplexes) of EGFP-encoding mRNA and pDNA. To determine the translation and transcription efficiency of each nucleic acid nanoplex, in vitro expression was measured in HEK293T7 cells that permit gene expression in the cytoplasmic region. Globally, mRNA… CONTINUE READING
BETA
1
Twitter Mention

Citations

Publications citing this paper.
SHOWING 1-3 OF 3 CITATIONS

References

Publications referenced by this paper.
SHOWING 1-10 OF 45 REFERENCES

Similar Papers

Loading similar papers…