Intra-specific variation withinEimeria tenella detected by the random amplification of polymorphic DNA

@article{Shirley1994IntraspecificVW,
  title={Intra-specific variation withinEimeria tenella detected by the random amplification of polymorphic DNA},
  author={Martin W. Shirley and Nat Bumstead},
  journal={Parasitology Research},
  year={1994},
  volume={80},
  pages={346-351}
}
The random amplification of polymorphic DNA (RAPD method), was used to derive fragments of DNA of the coccidial parasiteEimeria tenella for use both as genetic markers and as an aid for the discrimination of different wild-type and attenuated populations. Of 41 arbitrarily chosen 9-to 27-mer primers, 24 yielded useful arrays of fragments following low-stringency annealing conditions and the resultant profiles were generally very reproducible. One non-variant fragment of 2 kb hybridised to a… CONTINUE READING

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