Intra-axonal ferric ion-ferrocyanide staining of nodes of Ranvier and initial segments in central myelinated fibers.


Ferric ion and ferrocyanide were used to stain central nervous tissue from the spinal cords of rats following fixation in cacodylate-buffered aldehydes. At the nodes of Ranvier in myelinated fibers, the stain was localized primarily on the inner surface of the unmyelinated nodal axolemma, as had been reported previously for peripheral nodes. Unmyelinated… (More)