Intra- and extracellular measurement of reactive oxygen species produced during heat stress in diaphragm muscle.

@article{Zuo2000IntraAE,
  title={Intra- and extracellular measurement of reactive oxygen species produced during heat stress in diaphragm muscle.},
  author={Li Zuo and Fievos L Christofi and Valerie P Wright and Chang Ying Liu and Annamaria Merola and Lawrence J. Berliner and Thomas L Clanton},
  journal={American journal of physiology. Cell physiology},
  year={2000},
  volume={279 4},
  pages={
          C1058-66
        }
}
Skeletal muscles are exposed to increased temperatures during intense exercise, particularly in high environmental temperatures. We hypothesized that heat may directly stimulate the reactive oxygen species (ROS) formation in diaphragm (one kind of skeletal muscle) and thus potentially play a role in contractile and metabolic activity. Laser scan confocal microscopy was used to study the conversion of hydroethidine (a probe for intracellular ROS) to ethidium (ET) in mouse diaphragm. During a 30… CONTINUE READING
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