Interstrain pseudorecombinants of cowpea chlorotic mottle virus: effects on systemic spread and symptom formation in soybean and cowpea.

Abstract

Full-length complementary DNA (cDNA) copies of genomic RNA1, RNA2, and RNA3 segments of cowpea chlorotic mottle virus (CCMV) strains D, N, and S were synthesized using polymerase chain reaction and were cloned downstream of a T7 RNA polymerase promoter. Mixtures of the homologous in vitro-transcribed RNAs produced typical CCMV symptoms when inoculated on… (More)

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