Interspecific somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum

  title={Interspecific somatic hybrid plants between eggplant (Solanum melongena) and Solanum torvum},
  author={Asaf Guri and Kenneth C. Sink},
  journal={Theoretical and Applied Genetics},
Mesophyll protoplasts of eggplant (cv Black Beauty) and of Solanum torvum (both 2n=2x=24) were fused using a modification of the Menczel and Wolfe PEG/DMSO procedure. Protoplasts post-fusion were plated at 1 × 105/ml in modified KM medium, which inhibited division of S. torvum protoplasts. One week prior to shoot regeneration, ten individual calluses had a unique light-green background and were verified as cell hybrids by the presence of the dimer isozyme patterns for phosphoglucoisomerase (PGI… CONTINUE READING
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