Interspecific in vitro assay for the chimera-forming ability of human pluripotent stem cells.

Abstract

Functional assay limitations are an emerging issue in characterizing human pluripotent stem cells (PSCs). With rodent PSCs, chimera formation using pre-implantation embryos is the gold-standard assay of pluripotency (competence of progeny to differentiate into all three germ layers). In human PSCs (hPSCs), however, this can only be monitored via teratoma… (More)
DOI: 10.1242/dev.124016

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