Interspecific hybrids between Medicago sativa L. and annual Medicago containing Alfafa weevil resistance

  title={Interspecific hybrids between Medicago sativa L. and annual Medicago containing Alfafa weevil resistance},
  author={Yuko Mizukami and Mitsuru Kato and Tadashi Takamizo and Michio Kanbe and Susumu Inami and Kazumi Hattori},
  journal={Plant Cell, Tissue and Organ Culture},
Non-embryogenic protoplasts of Medicago rugosa and M. scutellata were electro-fused with iodoacetic acid-treated protoplasts of M. sativa (alfalfa). Putative somatic hybrid callus were obtained and some plants regenerated from both combinations. Hybridity of regenerants was confirmed by morphology, molecular means and cytological observations. Parental specific bands were recognized in somatic hybrids by Southern analysis. The somatic hybrids were perennial and their morphology was similar to M… CONTINUE READING


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