Interrogating the Escherichia coli cell cycle by cell dimension perturbations
@article{Zheng2016InterrogatingTE, title={Interrogating the Escherichia coli cell cycle by cell dimension perturbations}, author={Hai Zheng and Po-yi Ho and Meiling Jiang and B. G. Tang and Weirong Liu and Dengjin Li and Xuefeng Yu and Nancy Kleckner and Ariel Amir and Chenli Liu}, journal={Proceedings of the National Academy of Sciences}, year={2016}, volume={113}, pages={15000 - 15005} }
Significance How bacteria regulate cell division to achieve cell size homeostasis, with concomitant coordination of DNA replication, is a fundamental question. Currently, there exist several competing models for cell cycle regulation in Escherichia coli. We performed experiments where we systematically perturbed cell dimensions and found that average cell volume scales exponentially with the product of the growth rate and the time from initiation of DNA replication to the corresponding cell…
110 Citations
Two different cell-cycle processes determine the timing of cell division in Escherichia coli
- BiologyeLife
- 2021
The hypothesis that both chromosome replication and a replication-independent inter-division process can limit cell division is supported: the two processes have balanced contributions in non-perturbed cells, while width perturbations increase the odds of the replication- independent process being limiting.
Two different cell-cycle processes determine the timing of cell division in Escherichia coli
- BiologybioRxiv
- 2021
The hypothesis that both chromosome replication and a replication-independent inter-division process can limit cell division is supported: the two processes have balanced contributions in non-perturbed cells, while width perturbations increase the odds of the replication- independent process being limiting.
A general quantitative relation linking bacterial cell growth and the cell cycle
- Biology
- 2019
A single-cell division model is suggested, which not only reproduces the bulk relations observed but also recapitulates the adder phenomenon established recently for stochastically dividing cells13–15.
Cell size is coordinated with cell cycle by regulating initiator protein DnaA in E. coli.
- BiologyBiophysical journal
- 2020
Coordination between E. coli Cell Size and Cell Cycle Mediated by DnaA
- BiologybioRxiv
- 2020
The results suggest that the regulatory mechanisms of DnaA level and activity are associated with the invariance of initiation mass and the cell size general relationship for matching frequencies of replication initiation and cell division.
Genomewide phenotypic analysis of growth, cell morphogenesis, and cell cycle events in Escherichia coli
- Biology, Environmental ScienceMolecular systems biology
- 2018
An image‐based quantitative screen of the single‐gene knockout collection of Escherichia coli is described and many new genes involved in cell morphogenesis, population growth, nucleoid (bulk chromosome) dynamics, and cell division are identified.
High Osmolarity Modulates Bacterial Cell Size through Reducing Initiation Volume in Escherichia coli
- BiologymSphere
- 2018
This study quantitatively characterize the cell size and cell cycle of Escherichia coli cells growing exponentially under hyperosmotic stress, which is a common environmental stressor that profoundly affects the bacterial water content.
Spatio-temporal control of DNA replication by the pneumococcal cell cycle regulator CcrZ
- BiologybioRxiv
- 2019
A conserved and essential protein in pneumococci and related Firmicutes named CcrZ (for Cell Cycle Regulator protein interacting with FtsZ) is identified that couples cell division with DNA replication by controlling the activity of the master initiator of DNA replication, DnaA.
The bacterial cell cycle, chromosome inheritance and cell growth
- BiologyNature Reviews Microbiology
- 2019
This Review explores the contributions of chromosome structure and nucleoid organization to cell cycle regulation, detail the cellular processes involved in the initiation of DNA replication and DNA segregation and explore how those processes are linked to cell growth and cell division.
Threshold accumulation of a constitutive protein explains E. coli cell-division behavior in nutrient upshifts
- BiologyProceedings of the National Academy of Sciences
- 2021
This study interrogates dynamic size-growth behavior of single cells across nutrient upshifts with a high-precision microfluidic device and finds that the division rate changes quickly after nutrients change, much before growth rate goes to a steady state, and in a way that adder correlations are robustly conserved.
References
SHOWING 1-10 OF 60 REFERENCES
Simultaneous regulation of cell size and chromosome replication in bacteria
- BiologyFront. Microbiol.
- 2015
It is shown that both cell size and chromosome replication may be simultaneously regulated by the long-standing initiator accumulation strategy, which proposes that initiators are produced in proportion to the volume increase and is accumulated at each origin of replication, and chromosomes replication is initiated when a critical amount per origin has accumulated.
Single-Cell Analysis of Growth in Budding Yeast and Bacteria Reveals a Common Size Regulation Strategy
- BiologyCurrent Biology
- 2016
Cell Size and the Initiation of DNA Replication in Bacteria
- BiologyPLoS genetics
- 2012
The mechanisms responsible for coordinating DNA replication with cell size vary between these two model organisms, and the difference in behavior of the two bacteria appears to lie in the mechanisms that control the activity of DnaA.
Cell-Size Homeostasis and the Incremental Rule in a Bacterial Pathogen.
- BiologyBiophysical journal
- 2015
Chromosome Replication and the Division Cycle of Escherichia coli B/r
- BiologyJournal of bacteriology
- 1971
Within experimental errors, DNA content was dependent only on growth rate and independent of the type of culture, the carbon source, or the addition of growth factors.
Stochasticity and homeostasis in the E. coli replication and division cycle
- BiologyScientific reports
- 2015
The time between birth and initiation (B-period) compensates for stochastic variability in birth size and growth rate, and the time between termination and division (D-period), invalidating the notion that replication initiation is the principal trigger for cell division.
The initiation mass for DNA replication in Escherichia coli K‐12 is dependent on growth rate.
- BiologyThe EMBO journal
- 1994
The initiation mass of E. coli K‐12 cells is found to increase monotonically with decreasing growth rate, indicating that the control for timing of initiation is not governed by a direct connection between mass accumulation and the molecule(s) determining initiation of replication.