Interpretation of low-copy-number DNA profile after post-PCR purification

@article{Michel2009InterpretationOL,
  title={Interpretation of low-copy-number DNA profile after post-PCR purification},
  author={Sabine Michel and Anne De Bast and Isabelle Vandenbroere and Olivier Froment},
  journal={Forensic Science International: Genetics Supplement Series},
  year={2009},
  volume={2},
  pages={542-543}
}
  • S. Michel, A. Bast, O. Froment
  • Published 1 December 2009
  • Biology
  • Forensic Science International: Genetics Supplement Series
Utility of amplification enhancers in low copy number DNA analysis
TLDR
Investigation on low-quantity DNA samples of PCR additives, betaine, DMSO, PEG, and PCRboost®, found that betaine can improve amplification efficiency of LCN DNA samples, specifically by decreasing stutter in a dual locus system.
Fidelity of heterozygote balance and contributor proportions before and after post-PCR purification of low template two-person mixtures
TLDR
To determine if post-amplification purification of DNA from two- person LTDNA mixtures can improve profile interpretation, variable concentrations and mixture ratios of saliva extracts were applied to Macherey-Nagel NucleoSpin® and Qiagen MinElute® columns.
On DNA transfer: The lack and difficulty of systematic research and how to do it better.

References

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Simplified Low‐Copy‐Number DNA Analysis by Post‐PCR Purification
TLDR
Post‐PCR purification of the PCR product can increase the sensitivity of capillary electrophoresis to such an extent that DNA profiles can be obtained from <100 pg of DNA using 28‐cycle amplification.