Interpretation of Shotgun Proteomic Data

  title={Interpretation of Shotgun Proteomic Data},
  author={Alexey I. Nesvizhskii and Ruedi Aebersold},
The shotgun proteomic strategy based on digesting proteins into peptides and sequencing them using tandem mass spectrometry and automated database searching has become the method of choice for identifying proteins in most large scale studies. However, the peptide-centric nature of shotgun proteomics complicates the analysis and biological interpretation of the data especially in the case of higher eukaryote organisms. The same peptide sequence can be present in multiple different proteins or… CONTINUE READING