Internalized insulin receptors are recycled to the cell surface in rat hepatocytes.

  title={Internalized insulin receptors are recycled to the cell surface in rat hepatocytes.},
  author={M. Fehlmann and J. Carpentier and E Van Obberghen and Pierre Freychet and Peter Thamm and Derek Saunders and Dietrich Brandenburg and Lelio Orci},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={79 19},
We have followed the fate of cell surface insulin receptors in isolated rat hepatocytes by both a biochemical and a morphological approach. Hepatocytes were labeled with the photoreactive and biologically active 125I-labeled insulin analogue, [2-nitro-4-azidophenylacetylB2]des-PheB1-insulin, under conditions that allow for minimal internalization (2 hr at 15 degrees C). Analysis of the cell-associated radioactivity by NaDodSO4/polyacrylamide gel electrophoresis under reducing conditions… CONTINUE READING

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