Internalization of ouabain and replacement of sodium pumps in the plasma membranes of HeLa cells following block with cardiac glycosides.

  title={Internalization of ouabain and replacement of sodium pumps in the plasma membranes of HeLa cells following block with cardiac glycosides.},
  author={Joseph F Lamb and P H Ogden},
  journal={Quarterly journal of experimental physiology},
  volume={67 1},
  • J. LambP. Ogden
  • Published 22 January 1982
  • Biology
  • Quarterly journal of experimental physiology
The cardiac glycosides, ouabain or digoxin, when applied to HeLa cells at a concentration of 1 µM, block all Na/K pumping. The recovery of the cells from such a block involves a process of internalization of the glycoside, with its subsequent excretion and the appearance of a working Na pump at the plasma membrane. The rate of recovery of pump function probably does not depend on the direct dissociation of the glycoside from the sodium pumps on the plasma membrane. Low temperature and… 

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