Internalization of functional epidermal growth factor:receptor/kinase complexes in A-431 cells.

@article{Cohen1985InternalizationOF,
  title={Internalization of functional epidermal growth factor:receptor/kinase complexes in A-431 cells.},
  author={Stanley R. Cohen and Roy A. Fava},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 22},
  pages={12351-8}
}
We have isolated and partially purified an intracellular vesicle fraction from A-431 cells that contains both epidermal growth factor (EGF) and enzymatically active EGF:receptor/kinase. Exposure of intact A-431 cells to EGF leads to an accumulation of both EGF and kinase activity in this vesicle fraction. The accumulation is time- and temperature-dependent and is blocked by inhibitors of energy production. The EGF receptor in internalized vesicles is capable of autophosphorylation and, in the… CONTINUE READING