Internalization and release of insulin from hepatocytes

  title={Internalization and release of insulin from hepatocytes},
  author={Juan Carlos Cresto and Mar{\'i}a del Carmen Camberos and Viviana D’Alessandro and Juan Carlos Basabe},
  journal={Acta diabetologia latina},
Degradation of internalized insulin was studied after binding at 25 degrees C and 37 degrees C to isolated hepatocytes. The cells were washed to avoid extracellular insulin contamination. Degradation of both, intracellular and extracellular 125I-insulin, was measured with TCA and insulin antibody. In these conditions binding at 25 degrees C and 37 degrees C was equal but both intra and extracellular degradation were greater at 37 degrees C than at 25 degrees C. At both temperatures… CONTINUE READING


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