Internal transcribed spacer 2 amplicon as a molecular marker for identification of Peronospora parasitica (crucifer downy mildew).

@article{Casimiro2004InternalTS,
  title={Internal transcribed spacer 2 amplicon as a molecular marker for identification of Peronospora parasitica (crucifer downy mildew).},
  author={Sandra Casimiro and M{\'o}nica Moura and L{\'i}bia Z{\'e}-Z{\'e} and Rog{\'e}rio Tenreiro and Antonio A R Monteiro},
  journal={Journal of applied microbiology},
  year={2004},
  volume={96 3},
  pages={579-87}
}
AIMS The purpose of the study was to characterize the internal transcribed spacer (ITS) regions of Peronospora parasitica (crucifer downy mildew) in order to evaluate their potential as molecular markers for pathogen identification. METHODS AND RESULTS PCR amplification of ribosomal RNA gene block (rDNA) spacers (ITS1 and ITS2) performed in 44 P. parasitica isolates from different Brassica oleracea cultivars and distinct geographic origins, revealed no length polymorphisms. ITS restriction… CONTINUE READING

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