Intermolecular recombination assay for mammalian cells that produces recombinants carrying both homologous and nonhomologous junctions.

@article{Brouillette1987IntermolecularRA,
  title={Intermolecular recombination assay for mammalian cells that produces recombinants carrying both homologous and nonhomologous junctions.},
  author={Suzanne Brouillette and Pascal Chartrand},
  journal={Molecular and cellular biology},
  year={1987},
  volume={7 6},
  pages={2248-55}
}
We present an intermolecular recombination assay for mammalian cells that does not involve the reconstitution of a selectable marker. It is based on the generation of a shuttle vector by recombination between a bacterial and a mammalian vector. The recombinants can thus be amplified in mammalian cells, isolated by plasmid rescue in an Escherichia coli RecA- host, and identified by in situ hybridization, by using mammalian vector sequences as probes. Since both parental molecules can share… CONTINUE READING

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