Intermolecular duplexes in heterogeneous nuclear RNA from HeLa cells.

  title={Intermolecular duplexes in heterogeneous nuclear RNA from HeLa cells.},
  author={Nina Fedoroff and Peter K. Wellauer and Randolph Wall},
  volume={10 4},
Rapidly sedimenting hnRNA complexes contain regions of stable intermolecular duplex. Disruption of such complexes, as judged by a reduction in sedimentation rate, requires conditions sufficient to denature the duplex regions. Rapidly sedimenting molecules reappear only when the complementary sequences reanneal-that is, the formation of such complexes is dependent upon time and the concentration of homologous RNA. These experiments lead us to the conclusion that rapidly sedimenting hnRNA… CONTINUE READING