Intermolecular disulfide bond formation in the NEMO dimer requires Cys54 and Cys347.

@article{Herscovitch2008IntermolecularDB,
  title={Intermolecular disulfide bond formation in the NEMO dimer requires Cys54 and Cys347.},
  author={Melanie Herscovitch and William Comb and Thomas Ennis and Kate E Coleman and Sheila T. Yong and Brinda Armstead and Demetrios Kalaitzidis and Sushil A. Chandani and Thomas D. Gilmore},
  journal={Biochemical and biophysical research communications},
  year={2008},
  volume={367 1},
  pages={
          103-8
        }
}
NEMO is an essential regulatory component of the IkappaB kinase (IKK) complex, which controls activation of the NF-kappaB signaling pathway. Herein, we show that NEMO exists as a disulfide-bonded dimer when isolated from several cell types and analyzed by SDS-polyacrylamide gel electrophoresis under non-reducing conditions. Treatment of cells with hydrogen peroxide (H(2)O(2)) induces further formation of NEMO dimers. Disulfide bond-mediated formation of NEMO dimers requires Cys54 and Cys347… CONTINUE READING

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