Intermolecular complementation of the kinase activity of CheA.

  title={Intermolecular complementation of the kinase activity of CheA.},
  author={Ronald V. Swanson and Robert B. Bourret and Melvin I. Simon},
  journal={Molecular microbiology},
  volume={8 3},
CheA is a dimeric autophosphorylating protein kinase that plays a critical role in the signal transduction network controlling chemotaxis in Escherichia coli. The autophosphorylation reaction was analysed using mutant proteins defective in kinase and regulatory functions. Proteins in which the site of autophosphorylation was mutated (CheA48HQ) or missing (CheAs) were found to phosphorylate the kinase-defective mutant, CheA470GK. The kinetics of this reaction support the hypothesis that… CONTINUE READING

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