Intermolecular binding sites of human immunodeficiency virus type 1 Rev protein determined by protein footprinting.

@article{Jensen1995IntermolecularBS,
  title={Intermolecular binding sites of human immunodeficiency virus type 1 Rev protein determined by protein footprinting.},
  author={Torben Heick Jensen and Henrik Christian Leffers and J\orgen Kjems},
  journal={The Journal of biological chemistry},
  year={1995},
  volume={270 23},
  pages={13777-84}
}
Human immunodeficiency virus encodes the regulatory protein Rev, which is required for expression of viral structural proteins. It binds to an RNA element (RRE) in the viral transcript and up-regulates the cytoplasmic appearance of unspliced and singly spliced viral mRNA. We have studied the structure of Rev alone and complexed with the RRE and two monoclonal antibodies, using a protein footprinting approach. The method involves radioactive labeling at the C-terminal end of Rev fusion protein… CONTINUE READING