Intermolecular approach to metal ion indicators based on polymer phase transitions coupled to fluorescence resonance energy transfer.

@article{Yao2012IntermolecularAT,
  title={Intermolecular approach to metal ion indicators based on polymer phase transitions coupled to fluorescence resonance energy transfer.},
  author={Shaojun Yao and Aaron M. Jones and Jie Du and Randy K. Jackson and Justin O Massing and D. P. Kennedy and Nicholas E Bencivenga and Roy P. Planalp and Shawn Christopher Burdette and W. Rudolf Seitz},
  journal={The Analyst},
  year={2012},
  volume={137 20},
  pages={4734-41}
}
An approach to ratiometric fluorescence detection of quenching metal ions was devised by copolymerizing N-isopropylacrylamide with small percentages of bipyridine and amine monomers. The copolymer was divided into two portions. The amine group on one portion was functionalized with AlexaFluor555 (donor fluorophore) and the other with AlexaFluor647 (acceptor fluorophore). The indicator consists of a mixture of these two portions. Aggregation above the lower critical solution temperature (LCST… CONTINUE READING
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