Intermolecular H-Abstraction of Thiyl Radicals from Thiols and the Intramolecular Complexing of the Thiyl Radical with the Thiol Group in 1 , 4-Dithiothreitol . A Pulse Radiolysis Study

@inproceedings{Akhlaq2013IntermolecularHO,
  title={Intermolecular H-Abstraction of Thiyl Radicals from Thiols and the Intramolecular Complexing of the Thiyl Radical with the Thiol Group in 1 , 4-Dithiothreitol . A Pulse Radiolysis Study},
  author={Muhammad Akhlaq and Clemens von Sonntag},
  year={2013}
}
Hydroxyl radicals were generated by pulse radiolysis of N20-saturated aqueous solutions. The O H radicals react with thiols such as 1,4-dithiothreitol by abstracting S-bound H-atoms (k = 1.5 x 101() dm3 m ol“ 1 s_l). The 1,4-dithiothreitol radical HSCH2(C H O H )2CH2S' (2) closes the ring (k — 1.5 x 106 s“1) thereby forming the three-electron-bonded species Q H 2(C H O H )2C H 2S— $ H (3) in equilibrium (2, 3: e(380 nm) = 450 dm3 mol-1 cm-1) which has a reported pKa value of 5.2. A t pH 7.2 3… CONTINUE READING