Intermediates of recombination during mating type switching in Saccharomyces cerevisiae.

  title={Intermediates of recombination during mating type switching in Saccharomyces cerevisiae.},
  author={Charles I White and James E Haber},
  journal={The EMBO journal},
  volume={9 3},
We have identified two novel intermediates of homothallic switching of the yeast mating type gene, from MATa to MAT alpha. Following HO endonuclease cleavage, 5' to 3' exonucleolytic digestion is observed distal to the HO cut, creating a 3'-ended single-stranded tail. This recision is more extensive in a rad52 strain unable to switch. Surprisingly, the proximal side of the HO cut is protected from degradation; this stabilization depends on the presence of the silent copy donor sequences. A… CONTINUE READING

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