Intermediates in the refolding of ribonuclease at subzero temperatures. 1. Monitoring by nitrotyrosine absorbance.

Abstract

Derivatives of ribonuclease A in which tyrosines-73, -76, and -115 were nitrated have been synthesized, purified to homogeneity, and characterized by NMR, isoelectric points, absorbance spectra, and catalytic activity. The positions of their reversible thermal unfolding transitions were determined in 35% methanol at pH* 3.0 and 6.0. In the present study the… (More)

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@article{Biringer1988IntermediatesIT, title={Intermediates in the refolding of ribonuclease at subzero temperatures. 1. Monitoring by nitrotyrosine absorbance.}, author={Roger G. Biringer and A. L. Fink}, journal={Biochemistry}, year={1988}, volume={27 1}, pages={301-11} }