Intermediates in homologous pairing promoted by recA protein. Isolation and characterization of active presynaptic complexes.

@article{Tsang1985IntermediatesIH,
  title={Intermediates in homologous pairing promoted by recA protein. Isolation and characterization of active presynaptic complexes.},
  author={Siu Sing Tsang and K. Muniyappa and E. M. Azhderian and David K. Gonda and C. M. Radding and John Flory and J. W. Chase},
  journal={Journal of molecular biology},
  year={1985},
  volume={185 2},
  pages={
          295-309
        }
}
recA protein promotes homologous pairing and strand exchange by an ordered reaction in which the protein first polymerizes on single-stranded DNA. This presynaptic intermediate, which can be formed either in the presence or absence of Escherichia coli single-stranded binding protein (SSB), has been isolated by gel filtration and characterized. At saturation, purified complexes contained one molecule of recA protein per 3.6 nucleotide residues of single-stranded DNA. Complexes that had been… CONTINUE READING

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