Intermediates in extrachromosomal homologous recombination in Xenopus laevis oocytes: characterization by electron microscopy.

@article{PontKingdon1993IntermediatesIE,
  title={Intermediates in extrachromosomal homologous recombination in Xenopus laevis oocytes: characterization by electron microscopy.},
  author={Genevi{\`e}ve A. Pont-Kingdon and Robert J. Dawson and Dana Carroll},
  journal={The EMBO journal},
  year={1993},
  volume={12 1},
  pages={23-34}
}
Several molecular mechanisms have been proposed to account for nonconservative homologous recombination. This type of recombination is particularly efficient in Xenopus oocytes when appropriate DNA substrates are injected. To distinguish between possible models, we have investigated recombination intermediates from oocytes by direct observation in the electron microscope. Partially recombined DNA was crosslinked with a psoralen derivative after incubation in oocytes to limit the branch… CONTINUE READING