Intermediate purification of CHO-derived recombinant human Factor IX using hydrophobic interaction membrane-based chromatography and its comparison to a sulfated resin.

@article{Ribeiro2017IntermediatePO,
  title={Intermediate purification of CHO-derived recombinant human Factor IX using hydrophobic interaction membrane-based chromatography and its comparison to a sulfated resin.},
  author={Daniel A Ribeiro and Douglas F Passos and Helen Conceiç{\~a}o Ferraz and Leda R Castilho},
  journal={Electrophoresis},
  year={2017},
  volume={38 22-23},
  pages={2900-2908}
}
This work investigated the use of hydrophobic interaction membrane chromatography for intermediate purification of recombinant human Factor IX (rFIX) produced by CHO cells. The first purification step was based on a strong anion exchange monolith, thus forming a purification process fully based on convective media, which allow operation at high flow rates… CONTINUE READING