Intermediate formation at lower urea concentration in 'B' isomer of human serum albumin: a case study using domain specific ligands.

@article{Ahmad2004IntermediateFA,
  title={Intermediate formation at lower urea concentration in 'B' isomer of human serum albumin: a case study using domain specific ligands.},
  author={Basir Ahmad and Mohd Khursheed Alam Khan and Soghra Khatun Haq and Rizwan Hasan Khan},
  journal={Biochemical and biophysical research communications},
  year={2004},
  volume={314 1},
  pages={166-73}
}
The urea-induced unfolding of 'N' isomer (occurring at pH 7.0) and 'B' isomer (occurring at pH 9.0) of human serum albumin was studied by fluorescence and circular dichroism spectroscopic measurements. Urea-induced destabilization in different domains of both the isomers was monitored by using domain specific ligands, hemin (domain-I), chloroform, bilirubin (domain-II), and diazepam (domain-III). Urea-induced denaturation of N and B isomers of HSA showed a two-step, three-state transition with… CONTINUE READING