Interleukin 2-mediated immune interferon (IFN-gamma) production by human T cells and T cell subsets.


Human interleukin 2 (IL 2, or T cell growth factor), which was free of lectin and interferon activity (IFN), induced human peripheral T lymphocytes to produce immune IFN (IFN-gamma). In contrast, non-T cells and macrophages did not produce IFN-gamma in response to IL 2. IL 2 acted directly on unstimulated T cells to induce IFN-gamma production, and also acted in synergy with a suboptimal dose (2 micrograms/ml) of concanavalin A (Con A) to enhance IFN-gamma production. The IFN-gamma-inducing activity of partially purified IL 2 was absorbed along with the IL 2 activity by murine IL 2-dependent CT-6 cell line cells. This further supports the view that IFN-gamma-inducing activity is identical to IL 2. When T cells were separated further into helper/inducer T4+ and suppressor/cytotoxic T8+ subsets by negative selection with monoclonal antibody and complement, both T4+ and T8+-enriched cells produced significant levels of IFN-gamma in response to IL 2. Complete removal of macrophages from purified T lymphocyte populations by treatment of OKM1 plus complement consistently reduced IFN-gamma production in response to IL 2 to a limited degree; readdition of macrophages restored IFN-gamma production by both T cell subsets. This observation that IL 2 contributes to the production of IFN-gamma by human lymphocytes suggests that a cascade of lymphocyte-cell interactions participates in human immune responses.

Citations per Year

3,182 Citations

Semantic Scholar estimates that this publication has 3,182 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Kasahara1983Interleukin2I, title={Interleukin 2-mediated immune interferon (IFN-gamma) production by human T cells and T cell subsets.}, author={Tadashi Kasahara and John J. Hooks and Sean Dougherty and J. J. Oppenheim}, journal={Journal of immunology}, year={1983}, volume={130 4}, pages={1784-9} }