Interleukin-18 inhibits human immunodeficiency virus type 1 production in peripheral blood mononuclear cells.

Abstract

Interleukin (IL)-18 is an interferon (IFN)-gamma-inducing factor and contributes to the Th1 immune response. IL-18 added after infection of peripheral blood mononuclear cells (PBMC) with monocyte-tropic human immunodeficiency virus type 1 (HIV-1) inhibited p24 antigen production by a maximum of 72%. IFN-gamma levels in these cultures were increased, and a significant inverse relationship between HIV-1 production and IFN-gamma levels was observed. A neutralizing anti-IFN-gamma antibody reversed the IL-18 inhibitory effect. Preincubation of PBMC with IL-18 before infection inhibited p24 without additional IL-18 (64%). However, compared with the degree of IL-18 inhibition observed after a 4-day culture, no additional IL-18 inhibitory effect was observed during days 5-13. IL-18 also reduced cell surface expression of the HIV-1 receptor CD4. These results demonstrate that IL-18 inhibited HIV-1 production in PBMC through intermediate IFN-gamma. Furthermore, inhibition was present during the early stages of viral infection and was associated with reduced HIV-1 receptor expression.

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@article{Choi2001Interleukin18IH, title={Interleukin-18 inhibits human immunodeficiency virus type 1 production in peripheral blood mononuclear cells.}, author={Ha Jong Choi and Charles Anthony Dinarello and Leland Shapiro}, journal={The Journal of infectious diseases}, year={2001}, volume={184 5}, pages={560-8} }