Interlaboratory study on thermal cycler performance in controlled PCR and random amplified polymorphic DNA analyses.

@article{Saunders2001InterlaboratorySO,
  title={Interlaboratory study on thermal cycler performance in controlled PCR and random amplified polymorphic DNA analyses.},
  author={Graham C Saunders and James Dukes and Helen C. Parkes and Jack Cornett},
  journal={Clinical chemistry},
  year={2001},
  volume={47 1},
  pages={47-55}
}
BACKGROUND Intercomparisons of PCR-based data between laboratories require an assurance of assay reproducibility. We performed an interlaboratory study to investigate the contribution made by a variety of thermal cyclers to PCR performance as measured by interblock reproducibility and intrablock repeatability. METHODS Two standardized assays designed to minimize the introduction of non-thermal-cycler-dependent variations were evaluated by 18 laboratories in the United Kingdom, using 33… CONTINUE READING
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