Interlaboratory comparison of real-time PCR protocols for quantification of general fecal indicator bacteria.

@article{Shanks2012InterlaboratoryCO,
  title={Interlaboratory comparison of real-time PCR protocols for quantification of general fecal indicator bacteria.},
  author={Orin C. Shanks and Mano Sivaganesan and Lindsay Peed and Catherine A. Kelty and Angelia D Blackwood and Monica R Greene and Rachel T Noble and Rebecca N. Bushon and Erin A. Stelzer and Julie L. Kinzelman and Tamara Anan'eva and Christopher David Sinigalliano and David Wanless and John Griffith and Yiping Cao and Steve Weisberg and Valarie J Harwood and Christopher Staley and Kevin H. Oshima and Manju Varma and Richard A. Haugland},
  journal={Environmental science & technology},
  year={2012},
  volume={46 2},
  pages={945-53}
}
The application of quantitative real-time PCR (qPCR) technologies for the rapid identification of fecal bacteria in environmental waters is being considered for use as a national water quality metric in the United States. The transition from research tool to a standardized protocol requires information on the reproducibility and sources of variation associated with qPCR methodology across laboratories. This study examines interlaboratory variability in the measurement of enterococci and… CONTINUE READING