Interlaboratory comparison of polymerase chain reaction for the detection ofToxoplasma gondii DNA added to samples of amniotic fluid

@article{Guy1996InterlaboratoryCO,
  title={Interlaboratory comparison of polymerase chain reaction for the detection ofToxoplasma gondii DNA added to samples of amniotic fluid},
  author={Edward C. Guy and Herv{\'e} Pelloux and Maija Lappalainen and Horst Asp{\"o}ck and Andreas R. Hassl and Kjetil Klaveness Melby and M. Holberg-Pettersen and Eskild Petersen and Javier Sim{\'o}n and Pierre Ambroise-Thomas},
  journal={European Journal of Clinical Microbiology and Infectious Diseases},
  year={1996},
  volume={15},
  pages={836-839}
}
To investigate the accuracy of the polymerase chain reaction (PCR) method for the detection ofToxoplasma gondii in clinical specimens, aliquots of amniotic fluid to which known amounts ofToxoplasma gondii DNA had been added were tested by five European Centres. Four laboratories were able to detect DNA at levels equivalent to ten tachyzoites or less, including two that detected DNA equivalent to a single parasite. Two laboratories erroneously found one of eight negative control samples to be… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 19 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 12 references

Prenatal diagnosis of congenital toxoplasmosis with a polymerase chain reaction test on amniotic fluid

  • P Hohlfeld, F Daffos, Costa J-M, F ThulliezRForestier, M Vidaud
  • New England Journal of Medicine
  • 1994

Application of the polymerase chain reaction in the diagnosis of pulmonary toxoplasmosis in immunocompromised patients

  • A Roth, B Roth, G Hoffken, S Steuber, KI Khalifa, K Janitschke
  • European Journal of Clinical Microbiology…
  • 1992

Similar Papers

Loading similar papers…