Interlaboratory comparison of fetal male DNA detection from common maternal plasma samples by real-time PCR.

@article{Johnson2004InterlaboratoryCO,
  title={Interlaboratory comparison of fetal male DNA detection from common maternal plasma samples by real-time PCR.},
  author={Kirby L. Johnson and Kimberly A Dukes and John Vidaver and Erik S. Leshane and Idania Ramirez and William D. Weber and Farideh Z. Bischoff and Sinuhe Hahn and Arun Sharma and Dianne X Dang and Lisa M Hire and Diana W. Bianchi and Joe Leigh Simpson and Wolfgang Holzgreve and Sherman Elias and Katherine Wood Klinger},
  journal={Clinical chemistry},
  year={2004},
  volume={50 3},
  pages={516-21}
}
BACKGROUND Analysis of fetal DNA from maternal plasma by PCR offers great potential for noninvasive prenatal genetic diagnosis. To further evaluate this potential, we developed and validated a standard protocol to determine whether fetal DNA sequences could be reproducibly amplified and measured across multiple laboratories in a common set of specimens. METHODS Each of five participating centers in a National Institute of Child Health and Human Development consortium collected 20 mL of… CONTINUE READING