Interferon-Like Virus-Inhibitor Induced in Human Leukocytes by Phytohemagglutinin

@article{Wheelock1965InterferonLikeVI,
  title={Interferon-Like Virus-Inhibitor Induced in Human Leukocytes by Phytohemagglutinin},
  author={E. Frederick Wheelock},
  journal={Science},
  year={1965},
  volume={149},
  pages={310 - 311}
}
Phytohemagglutinin, an extract of the kidney bean, Phaseolus vulgaris, induces in human leukocyte cultures an inhibitor of the cytopathic effects of Sindbis virus. The physicochemical and biological properties of this virus-inhibitor are similar to those of interferon induced by Newcastle disease virus, except for an instability at pH 2 and 10 and at 56�C. 

An interferon-like inhibitor of foot-and-mouth disease virus induced by phytohemagglutinin in swine leukocyte culturesb

  • J. Richmond
  • Biology, Medicine
    Archiv für die gesamte Virusforschung
  • 2005
An inhibitor of foot-and-mouth disease virus was produced in cultures of swine leukocytes following treatment with phytohemagglutinin and the biological and physical properties suggest it has many properties similar to those of an interferon.

Interferons of foot-and-mouth disease virus: A new assay for interferon

  • J. Richmond
  • Biology
    Archiv für die gesamte Virusforschung
  • 2005
Phytohemagglutinin induction of an interferon effective against foot-and-mouth disease virus was a property of bovine as well as swine peripheral blood leukocytes in suspension cultures and plaque-size measurements suggested a possible new assay system for interferons.

Vaccinia Virus Replication and Cytopathic Effect in Cultures of Phytohemagglutinin-treated Human Peripheral Blood Leukocytes

Increase in viral titer was accompanied by cell injury, decline in cell numbers, and decreased acid production, and it was found that leukocytes not pretreated with phytohemagglutinin appeared to form infective centers after exposure to the vaccinia agent, but they did not replicate infectious virus.

Interferon inhibits DNA synthesis induced in mouse lymphocyte suspensions by phytohaemagglutinin or by allogeneic cells.

The inhibition by interferon of DNA synthesis induced in mouse spleen lymphocytes by the non-viral stimuli phytohaemagglutinin (PHA) and allogeneic lymphocytes is reported, suggesting that by acting on lymphocytes, interferons plays a role in the immunological response of the host.

Induction of interferon in ovine and human lymphocyte cultures by mycoplasmas

The antiviral substance induced by the mycoplasmas in human lymphocytes was characterized as interferon by the usual criteria and produced significant levels of interferons in response to infection with M. pneumoniae and M. synoviae.

Biosynthetic human interferon treatment of herpetic keratitis.

  • G. Smolin
  • Biology, Medicine
    Transactions of the American Ophthalmological Society
  • 1983
All interferons induce in cells a resistance to a broad spectrum of viruses, used to measure the biologic activity of the interferon (IF).

Host Cells for Vesicular Stomatitis Virus Replication In Vitro

The treatment of PHA with heat, digestive enzymes, rabbit anti-PHA serum and serial dilutions failed to dissociate that portion of the PHA extract responsible for the conversion of lymphocytes into virus-susceptible cells from those components responsible for leukoagglutination or lymphocyte transformation.

T-cell hybridoma-produced lymphokine that activates macrophages to suppress intracellular growth of Histoplasma capsulatum

Supernatants from concanavalin A-stimulated T-cell hybridomas activated macrophages to suppress the intracellular growth of Histoplasma capsulatum and to kill tumor cells and Gamma interferon is suggested to be among the active substances in the supernatants.

Gamma Interferon Is a Major Suppressive Factor Produced by Activated Human Peripheral Blood Lymphocytes That Is Able To Inhibit Foamy Virus-Induced Cytopathic Effects

Antibodies against gamma interferon (IFn-γ) are able to suppress most of the inhibitory activity, suggesting that IFN-γ is the dominant component in the foamy virus-induced cytopathic effects in vitro.

Immune human lymphocytes produce an acid-labile α-interferon

A novel human interferon with antigenic and cross-species reactivity of α- IFN and physicochemical properties of γ-IFN is described and will be useful in elucidating the biological significance and cell of origin of this IFN.
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