• Corpus ID: 42975511

Interference of UDP-glucuronyltransferase and beta-glucuronidase activity in rat liver microsomes at pH 7.5 with p-nitrophenol and p-nitrophenylglucuronide as substrates.

@article{Gigon1979InterferenceOU,
  title={Interference of UDP-glucuronyltransferase and beta-glucuronidase activity in rat liver microsomes at pH 7.5 with p-nitrophenol and p-nitrophenylglucuronide as substrates.},
  author={Philippe Gigon and MARCEL H. Bickel},
  journal={Enzyme},
  year={1979},
  volume={24 4},
  pages={
          230-8
        }
}
  • P. Gigon, M. Bickel
  • Published 1979
  • Biology, Medicine, Computer Science, Chemistry
  • Enzyme
Microsomal fraction contains the whole of hepatic UDP-glucuronyltransferase as well as part of beta-glucuronidase. The activities of the two enzymes were assayed under identical conditions using untreated male rat liver microsomes at pH 7.5. In a 30-min incubation with p-nitrophenol and UPD-glucuronic acid, a net glucuronide formation of 0.010 mumol.min-1.g.liver-1 was measured. In the presence of saccharolactone at concentrations selectively blocking beta-glucuronidase, the glucuronidation… 
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This textbook specifically focuses on kinetics of drug-metabolizing enzymes, detailing specific enzyme classes, and discusses kinetics as they apply to drug transporters and additional factors such as variability in isoforms and experimental factors are outlined.